Interests and Expertise:
​Aquaculture, Biochemistry, Climate Change and Physiology.

Tiago Repolho, Junior Researcher

Tiago Repolho (TR) is an integrated researcher at the Marine and Environmental Sciences Centre (MARE, Portugal) and an invited senior lecturer at the Department of Animal Biology (FCUL, Uni. Lisbon, Portugal). He graduated in Biology (Uni. Aveiro, 2001), obtained an MSc in Biology and Marine Resources Management (FCUL, 2007) and a PhD in Marine Biology and Aquaculture (FCUL, 2012). TR was a postdoctoral researcher at MARE/FCUL, where he studied the acclimation and adaptation potential of marine biota to environmental drivers (ocean warming, ocean acidification). Since the early start of his career, TR has been gathering (national and international level) a strong multidisciplinary scientific expertise in several research fields (aquaculture, biochemistry, ecotoxicology, fisheries, global change biology, physiology, seafood safety). He is passionate about Aquaculture, Environmental, and Marine Sciences research. Taking advantage of his multidisciplinary background, TR aims to synergistically bridge these areas and his core expertise towards developing an emerging, climate-resilient and environmentally-friendly green technology, i.e. marine aquaponics (MA). Besides developing an innovative MA research line at his host and delivering novel empirical based-knowledge and technological advancements in MA research, TR intends to further support future decision-making strategies (aquaculture development, CC mitigation, env. sustainability, blue growth) in alignment with the UN 2030 agenda for sustainable development (SDGs 9&14), the EU strategy for blue growth, and the EU green deal initiative. Since 2002, TR has acted as PI (n=4), Co-PI (n=1) and senior team member in 24 national and international R&D funded projects (e.g. ERDF, FP5, EMFF, FCT, PADI foundation), besides being a PI (n=2) of additional competitive research grants (Assemble+, Company of Biologists). He has published 42 peer-review articles [H-index: 24, citations: 1372]. TR has presented 54 oral (4 by invitation) and 44 poster communications in national and international meetings. He has received national and international recognition from peers through scientific awards, grants, travelling fellowships, national and international appointments. Currently, TR is a scientific committee member of MARE (since 2019), scientific officer for SCUBA diving activities at LMG (MARE, since 2019) and elected member of the FCUL animal welfare ruling body (since 2021). Internationally, TR is a member of Frontiers in Mar. Science editorial board, leading guest editor of a special issue (Biology, MDPI), member of the Society for Open, Reliable, and Transparent Ecology and Evolutionary Biology (SORTEE) and member of the Global Ocean Acidification Observing Network (GOA-ON). Additionally, TR acts as an invited reviewer for international publishers (peer-reviewed journals, book proposals, since 2011) and as evaluator and rapporteur (biological and environmental sciences) to prestigious national (ANI) and international organizations (e.g. US National Science Foundation, Dutch Research Council, EU REA, since 2016). Besides his recent appointment as a senior invited lecturer (MSc in Marine Ecology, FCUL, 2021), he has also been an invited lecturer within national academic institutions (MSc and BSc courses. FCUL, Uni. of Aveiro, Inst. Politécnico Santarém, since 2004). Currently, TR supervises 1 PhD student, 2 MSc students and 1 MSc research scholar. He provides advanced course training to PhD students (doctoral programs in Biology and Marine Sciences, FCUL, since 2015) and experimental tutoring to MSc and PhD students (since 2012). Currently, his international research network spreads over Europe, the USA, Australia and South America. His commitment is also science outreach-oriented (e.g. 52 school outreach activities since 2016). He is an objective-driven professional, always looking to undertake new professional challenges and opportunities academically and privately.

Academic Qualifications
2012.  Ph.D. in Marine Biology and Aquaculture, Faculty of Sciences, University of Lisbon , Portugal

2007. MSc in Biology and Marine Resource Management, Faculty of Sciences, University of Lisbon, Portugal
​
2001. BSc in Biology, University of Aveiro, Portugal.

Awards and Grants
2018. Outstanding Reviewing Contribution - Science of the Total Environment journal
2018. Outstanding Reviewing Contribution - Ecotoxicology and Environmental Safety journal
2017. Recognized reviewer - Ecotoxicology and Environmental Safety journal
2017. Recognized Reviewer - Science of the Total Environment journal
2016. Travelling Fellowship - Journal of Experimental Biology / Company of Biologists
2016. Travelling Fellowship  - 4th International Symposium on the Ocean in a High-CO2 World (Hobart, Tasmania)
2014. Travelling Fellowship (Sven Lovén Centre for Marine Sciences, University of Gothenburg, Sweden)
2014. Team-spirit Award (Rui Rosa Lab, MARE-Marine and Environmental Sciences Centre, Portugal)
2014. Best Oral Group Presentation (CeMEB Advanced Course: Marine Evolution under Climate Change, Sven Lovén Centre for Marine Science, University of Gothenburg, Sweden)
2013. Postdoctoral Fellowship. Portuguese Foundation for Science and Technology (FCT, Portugal)
2008. PhD scholarship. Portuguese Foundation for Science and Technology (FCT, Portugal)
2001. Travelling Fellowship "Oceanographic Training course" (Baltic Floating University, Student Mobility Action, T-JEP-10814-99). Intergovernmental Oceanographic Commission (IOC)​
2000. Erasmus+ scholarship (Department of Fisheries and Marine Biology, Department of Molecular Biology, University of Bergen, Norway)

Publications
THESIS

2012. Repolho T. Evaluation of Paracentrotus lividus (Lamarck, 1816) exotrophic larvae as live feed for marine decapod crustacean larvae. PhD Thesis. Faculty of Sciences, University of Lisbon

2007. Repolho T.  Effect of sea urchin Paracentrotus lividus (Lamarck, 1816) broodstock diet on larvae development. MSc Thesis. Faculty of Sciences, University of Lisbon.

2001. Repolho T. Intra and Inter-individual ITS variation in cod (Gadus morhua) reared under identical (light/feeding) and different water temperature (8°, 12° and 15°C) conditions: Inter-species comparative analysis with other teleost fishes. BSc Thesis. University of Aveiro, Portugal / University of Bergen, Norway

PUBLICATIONS IN INTERNATIONAL PEER-REVIEWED JOURNALS
  1. Clutton, E.A., Alurralde, G. & Repolho, T. (2021). Early developmental stages of native populations of Ciona intestinalis under increased temperature are affected by local habitat history. Journal of Experimental Biology, 224: jeb.233403 https://doi.org/10.1242/jeb.233403
  2. Otjacques, E., Repolho, T., Paula, J.R., Simão, S., Baptista, M. & Rosa, R. (2020). Cuttlefish buoyancy control in response to food availability and ocean acidification. Biology, 9 (7): 147 https://doi.org/10.3390/biology9070147
  3. Corriere, M., Baptista, M., Paula, J.R., Repolho, T., Rosa, R., Costa, P.R. & Soliño, L. (2020). Impaired fish swimming performance following dietary exposure to the marine phycotoxin okadaic acid. Toxicon, 179: 53-59. https://doi.org/10.1016/j.toxicon.2020.02.022
  4. Paula, J.R., Baptista, M., Carvalho, F., Repolho, T., Bshary, R. & Rosa, R. (2019). The past, present and future of cleaner fish cognitive performance as a function of CO2 levels. Biology Letters, 15: 20190618. https://doi.org/10.1098/rsbl.2019.0618
  5. Paula, J.R., Repolho, T., Pegado, M., Thörnqvist, P-O, Bispo, R., Winberg, S., Munday, P.L. & Rosa, R. (2019). Neurobiological and behavioural responses of cleaning mutualisms to ocean warming and acidification. Scientific Reports, 9: 12728. https://doi.org/10.1038/s41598-019-49086-0
  6. Rosa, R., Santos, C., Lopes, A., Baptista, M., Sampaio, E., Rosa, I. C., & Repolho, T. (2019). Stenophagy as a Pathway for the Occurrence of Unique Coral Fatty Acid Biomarkers in Higher Branches of the Marine Tree of Life: The Nudibranch Armina maculata Case. Ocean Science Journal, 54: 297-305. https://doi.org/10.1007/s12601-019-0004-z
  7. Grilo, T. F., Repolho, T., Rosa, R., & Cardoso, P. G. (2019). Performance and herbivory of the tropical topshell Trochus histrio under short-term temperature increase and high CO2. Marine Pollution Bulletin, 138: 295-301. https://doi.org/10.1016/j.marpolbul.2018.11.011
  8. Araújo, J.E., Madeira, D., Vitorino, R., Repolho, T., Rosa, R. & Diniz, M., (2018). Negative synergistic impacts of ocean warming and acidification on the survival and proteome of the commercial sea bream, Sparus aurata. Journal of Sea Research, 139: 50-61. https://doi.org/10.1016/j.seares.2018.06.011
  9. Lopes, A.R., Faleiro, F., Rosa, I.C., Pimentel, M.S., Trubenbach, K., Repolho, T., Diniz, D. & Rosa, R. (2018). Physiological resilience of a temperate soft coral to ocean warming and acidification. Cell Stress and Chaperones, 23 (5): 1093-1100. https://doi.org/10.1007/s12192-018-0919-9
  10. Dionísio, G., Faleiro, F., Bispo, R., Lopes, A., Cruz, S., Paula, J.R., Repolho, T., Calado, R. & Rosa, R. (2018). Distinct bleaching resilience of photosynthetic plastid-bearing mollusks under thermal stress and high CO2conditions. Frontiers in Physiology, 9: 1675. https://doi.org/10.3389/fphys.2018.01675
  11. Sampaio, E., Lopes, A.R., Francisco, S., Paula, J.R., Pimentel, M., Maulvault, A.L., Repolho, T., Grilo, T.F., Pousão-Ferreira, P., Marques, A. & Rosa, R. (2018). Ocean acidification dampens physiological stress response to warming and contamination in a commercially-important fish (Argyrosomus regius). Science of the Total Environment, 618: 388-398. https://doi.org/10.1016/j.scitotenv.2017.11.059
  12. Jesus, T.F., Rosa, I.C., Repolho, T., Lopes, A.R., Pimentel, M.S., Almeida-Val, V.M.F., Coelho, M.M. & Rosa, R. (2018). Different ecophysiological responses of freshwater fish to warming and acidification. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 216: 34-41. https://doi.org/10.1016/j.cbpa.2017.11.007
  13. Jesus, T.F., Moreno, J.M., Repolho, T., Athanasiadis, A., Rosa, R., Almeida-Val, V.M.F. & Coelho, M.M. (2017). Protein analysis and gene expression indicate differential vulnerability of Iberian fish species under a climate change scenario. PLoS ONE, 12 (7): e0181325. https://doi.org/10.1371/journal.pone.0181325
  14. Maulvault, A.L., Barbosa, V., Alves, R., Custódio, A., Anacleto, P., Repolho, T., Ferreira, P.P., Rosa, R., Marques, A. & Diniz, M. (2017). Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to increased temperature and dietary methylmercury. Science of the Total Environment, 586: 551-558. https://doi.org/10.1016/j.scitotenv.2017.02.016
  15. Repolho, T., Duarte, B., Dionísio, G., Lopes, A.M., Grilo, T., Paula, J.R., Rosa, I.C., Calado, R., Caçador, I. & Rosa, R. (2017). Seagrass ecophysiological performance under ocean warming and acidification. Scientific Reports, 7: 41443. https://doi.org/10.1038/srep41443 
  16. Rosa, R., Paula, J.R., Sampaio, E., Pimentel, Lopes, A.R., Baptista, M., Guerreiro, M., Santos, C., Campos, D., Almeida-Val, V.M.F., Calado, R., Diniz, M. & Repolho, T. (2016). Neuro-oxidative damage and aerobic potential loss of sharks under climate change. Marine Biology, 163: 119. https://doi.org/10.​1007/​s00227-016-2898-7 
  17. Sampaio, E., Maulvault, A.L., Lopes, V.M., Paula, J.R., Barbosa, V., Alves, R., Pousão-Ferreira, P., Repolho, T.,Marques, A. & Rosa, R. (2016). Habitat selection disruption and lateralization impairment of cryptic flatfish in a warm, acid, and contaminated ocean. Marine Biology, 163: 217. https://doi.org/10.​1007/​s00227-016-2994-8
  18. Maulvault, A.L., Custódio, A., Anacleto, P., Repolho, T., Pousão, P., Nunes, M.L., Diniz, M., Rosa, R. & Marques, A. (2016). Bioaccumulation and elimination of mercury in juvenile seabass (Dicentrarchus labrax) in a warmer environment. Environmental Research, 149: 77-85. https://doi.org/10.1016/j.envres.2016.04.035
  19. Rosa, R., Pimentel, M., Galan, J.G., Baptista, M., Lopes, V.M., Couto, A., Guerreiro, M., Sampaio, E., Castro, J., Santos, C., Calado, R. & Repolho, T. (2016). Deficit in digestive capabilities of bamboo shark early stages under climate change. Marine Biology, 163: 60. https://doi.org/10.1007/s00227-016-2840-z
  20. Faleiro, F., Santos, C., Baptista, M., Pimentel, M., Aurélio, M., Pegado, M.R., Calado, R., Repolho, T. & Rosa, R. (2015). Seahorses under a changing ocean: the impact of warming and acidification on a poor swimmer bony-armoured fish. Conservation Physiology, 3 (1): COV009. https://doi.org/10.1093/conphys/cov009
  21. Anacleto, P., Maulvault, A.L., Bandarra, N.M., Repolho, T., Nunes, M.L., Rosa, R. & Marques, A. (2014). Effect of warming on protein, glycogen and fatty acid content of native and invasive clams. Food Research International, 64: 439-445. https://doi.org/10.1016/j.foodres.2014.07.023
  22. Rosa, R., Baptista, M., Lopes, V.M., Pegado, M.R., Leal, M.C., Calado, R. & Repolho, T. (2014). Early-life exposure to climate change impairs tropical shark survival. Proceedings of the Royal Society B: Biological Sciences, 281: 20141738. https://doi.org/10.1098/rspb.2014.1738
  23. Rosa, R., Lopes, A., Pimentel, M., Faleiro, F., Baptista, M., Trübenbach, K., Narciso, L., Dionísio, G., Pegado, M.R., Repolho, T., Calado, R. & Diniz, M. (2014). Ocean’s cleaning stations under a changing climate: biological responses of tropical and temperate fish-cleaner shrimps to global warming. Global Change Biology, 20 (10): 3068-3079. https://doi.org/10.1111/gcb.12621
  24.  Pimentel, M., Faleiro, F., Dionísio, G., Repolho, T., Pousão-Ferreira, P., Machado, J. & Rosa, R. (2014). Defective skeletogenesis and oversized otoliths in fish early stages in a changing ocean. Journal of Experimental Biology, 217: 2062-2070. https://doi.org/10.1242/jeb.092635
  25. Rosa, R., Trübenbach, K., Pimentel, M.S., Portugal, J.B., Faleiro, F., Baptista, M., Dionísio, G., Calado, R, Pörtner, H.O. & Repolho, T. (2014). Differential impacts of ocean acidification and warming on winter and summer progeny of a coastal squid (Loligo vulgaris). Journal of Experimental Biology, 217: 518-525. https://doi.org/10.1242/jeb.096081
  26. Anacleto, P., Maulvault, A., Lopes, V.M., Repolho, T., Diniz, M., Nunes, M., Marques, A. & Rosa, R. (2014). Ecophysiology of native and alien-invasive clams in an ocean warming context. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 175: 28–37. https://doi.org/10.1016/j.cbpa.2014.05.003
  27. Baptista, M., Repolho, T., Maulvault, A.L., Lopes, V.M., Narciso, L., Marques, A., Bandarra, N. & Rosa, R. (2014). Temporal dynamics of amino and fatty acid composition in the razor clam Ensis siliqua (Mollusca: Bivalvia).Helgoland Marine Research, 68: 465-482. https://doi.org/10.1007/s10152-014-0402-7
  28. Pimentel, M.S., Pegado, M.R., Repolho, T. & Rosa, R. (2014). Impact of ocean acidification in the metabolism and swimming behavior of the dolphin fish (Coryphaena hippurus) early larvae. Marine Biology, 161 (3): 725-729.https://doi.org/10.1007/s00227-013-2365-7
  29. Lopes, V.M, Baptista, M., Repolho, T., Rosa, R. & Costa, P.R. (2014). Uptake, transfer and elimination kinetics of paralytic shellfish toxins in common octopus (Octopus vulgaris). Aquatic Toxicology, 146: 205–211. https://doi.org/10.1016/j.aquatox.2013.11.011
  30. Repolho, T., Baptista, M., Pimentel, M.S., Dionísio, G., Trübenbach, K., Lopes, V.M., Lopes, A.R., Calado, R., Diniz, M. & Rosa, R. (2014). Developmental and physiological challenges of octopus (Octopus vulgaris) early life stages under ocean warming. Journal of Comparative Physiology B, 184 (1): 55-64. https://doi.org/10.1007/s00360-013-0783-y
  31. Aurélio, M., Faleiro, F., Lopes, V.M., Pires, V.L., Lopes, A.R., Pimentel, M.S., Repolho, T., Narciso, L. & Rosa, R. (2013). Physiological and behavioral responses of temperate seahorses (Hippocampus guttulatus) to environmental warming. Marine Biology, 160 (10): 2663-2670. https://doi.org/10.1007/s00227-013-2259-8
  32. Rosa, R., Trübenbach, K., Repolho, T., Pimentel, M.S., Faleiro, F., Boavida-Portugal, J., Baptista M., Dionísio, G., Leal, M., Calado, R. & Pörtner, H.-O. (2013). Lower hypoxia thresholds of early life stages living in a warm acidified ocean. Proceedings of the Royal Society B: Biological Sciences, 280 (1768): 20131695. https://doi.org/10.1098/rspb.2013.1695
  33. Lopes, A.R., Trübenbach, K., Teixeira, T., Lopes, V.M., Pires, V., Baptista, M., Repolho, T., Calado, R., Diniz, M. &Rosa, R. (2013). Oxidative stress in deep scattering layers: heat shock response and antioxidant enzymes activities of myctophid fishes thriving in oxygen minimum zones. Deep Sea Research Part I: Oceanographic Research Papers, 82: 10-16. https://doi.org/10.1016/j.dsr.2013.07.014
  34. Pimentel, M.S., Trübenbach, K., Faleiro, F., Portugal, J.B., Repolho, T. & Rosa, R., (2012). Impact of ocean warming on the early ontogeny of cephalopods: a metabolic approach. Marine Biology, 159 (9): 2051-2059.https://doi.org/10.1007/s00227-012-1991-9
  35. Lopes, V.M., Baptista, M., Pimentel, M.S., Repolho, T., Narciso, L. & Rosa, R. (2012). Reproduction in Octocorallia: Synchronous spawning and asynchronous oogenesis in the pennatulid Veretillum cynomorium. Marine Biology Research, 8 (9): 1-10. https://doi.org/10.1080/17451000.2012.693616
  36. Repolho, T., Costa, M.H., Luís, O.J. & Gago, J. (2011). Broodstock diet effect on sea urchin Paracentrotus lividus(Lamarck, 1816) endotrophic larvae development: Potential for their year-round use in environmental toxicology assessment. Ecotoxicology and Environmental Safety, 74 (4): 584-592. https://doi.org/10.1016/j.ecoenv.2010.12.004
  37. Gago, J.M., Luís, O.J. & Repolho, T. (2009). Fatty acid nutritional quality of sea urchin Paracentrotus lividus(Lamarck, 1816) eggs and endotrophic larvae: relevance for feeding of marine larval fish. Aquaculture Nutrition, 15 (4): 379-389. https://doi.org/10.1111/j.1365-2095.2008.00602.x
  38. Caeiro, S., Costa, M.H., DelValls, A., Repolho, T., Gonçalves, M., Mosca, A., Coimbra, A.P., Ramos, T.B. & Painho, M. (2009). Ecological risk assessment of sediment management areas: application to Sado Estuary, Portugal. Ecotoxicology, 18 (8): 1165-1175. https://doi.org/10.1007/s10646-009-0372-8
  39. Costa, P.M., Repolho, T., Caeiro, S., Diniz, M.E., Moura, I. & Costa, M.H. (2008). Modelling metallothionin induction in the liver of Sparus aurata exposed to metal-contaminated sediments. Ecotoxicology and Environmental Safety, 71 (1): 117-124. https://doi.org/10.1016/j.ecoenv.2007.05.012
  40. Johansen, T., Repolho, T., Hellebo, A. & Raae, A.J. (2006). Strict conservation of the ITS regions of the ribosomal RNA genes in Atlantic cod (Gadus morhua L.). DNA Sequence, 17 (2): 107-114. https://doi.org/10.1080/10425170600624701
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